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The protein expression levels of tight junction associated protein-1 (ZO-1), Occludin, inflammatory factors interleukin (IL)-6, IL-1 and tumor necrosis factor (TNF)-, SIRT1, STAT3, p-STAT3, and retinoic acid receptor-related orphan receptor gamma t (RORt) were detected by Western blot

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In mixed urine samples collected between 8 and 72 h, the proportion of tritium water was higher, accounting for 69.5% (female) and 75.3% (male) of the total radioactivity, and [ 3 H]proline (M1) accounted for 3.11% (female) and 4.17% (male) of the total radioactivity (Figure 5B)

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